System Requirements ------------------------------------------------------------------------------ 1. OS Platform:- Linux 2. Perl 4.0, or above Required Inputs ----------------------------------------------------------------------------- 1. Gff file (.gff) 2. Corresponding Genome file in fasta format. Inputs Example Files ----------------------------------------------------------------------------- 1. Gff File: /GFF/example.gff 2. Genome: /GFF/sequence.fasta Output Preffix(Feature Name)_Suffix(Input GFF/Genome) ----------------------------------------------------------------------------- 1. gff file wth unique ID i.e their Sr. Nos. - OUTPUT: ID_(input "gff" filename) 1. Gene Sequences - OUTPUT: gene_(input "genome" filename) 2. Exon Sequences - OUTPUT: exon_(input "genome" filename) 3. CDS/cDNA Sequences - OUTPUT: CDS_(input "genome" filename) 4. mRNA Sequences - OUTPUT: mRNA_(input "genome" filename) 5. Intron Sequences - OUTPUT: intron_(input "genome" filename) 6. Intergenic Sequences - OUTPUT: intergenic_(input "genome" filename) 7. Promoter / Gene Upstream Sequences - OUTPUT: upstream_(input "genome" filename) Note: The output sequences will be in fasta format and the header of each seqeunce will state the Sr. No. or Unique IDs preffixed with the plugin name (eg. gene_20). Unique IDs/Sr. No. will be in accordance to the IDs/Sr. No.(first column) in the Unique-ID file [OUTPUTFILE: ID_(input "gff" filename]. Run ----------------------------------------------------------------------------- 1. Download GFF.tar.gz 2. sudo -s 3. gunzip GFF.tar.gz 4. tar -vxf GFF.tar 5. cd GFF 6. perl permissions.pl ------- give permissions to the executable files 7. ./gff.sh ------- will execute the whole progrram Acknowledgements ------------------------------------------------------------------------------ 1. Dr. Dinesh Gupta 2. Mr. Abhinav Kaushik Developerz Information ------------------------------------------------------------------------------ Achal Rastogi (Dr. Dinesh Gutpa Group) Junior Research Fellow Bioinformatics Facility Structural & Computational Biology Laboratory International Center for Genetic Engineering And Biotechnology Aruna Asaf Ali Marg, New Delhi 110067 India Email: achal@icgeb.res.in NOTES ------------------------------------------------------------------------------ 1. Changing the syntax of any script can make the program unexecutable or can result in wrong fetches. 2. Take care of input directories path while giving an input. (OR) keep the input file in program directory itself.