System Requirements
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1. OS Platform:- Linux
2. Perl 4.0, or above
3. GFF-Ex_v2.1

Required Input Files
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1. A tab-delimited multi-column file.
2. Columns numbers to fecth the desired column information.
3. Sequence file in fasta format. (.fa, .fasta, fna, etc.)


Inputs Example Files
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1. Multi-column File	/COLx/examples/input

2. Desired Columns	START	END
			9	10		

3. Sequence File:	/GFF_v2.1/examples/sequence.fasta


Output Files													Preffix(Plugin Name)_Suffix(Input multicolumnfile/sequencefile)
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1. Multi-column file wth unique ID i.e their Sr. Nos.					- OUTPUTFILE:	ID_(input "multi-column" filename).gff
2. Desired Sequences									- OUTPUTFILE:	colx_(input "genome" filename)

Note: The output sequences will be in fasta format and the header of each seqeunce will state the Serial Number or Unique IDs preffixed with the plugin name (eg. Colx_1). Unique IDs/Serial Number will be in accordance to the IDs/Serial Number (first column) in the Unique-ID file [OUTPUTFILE: ID_(input "multi-column" filename).gff].

Installation
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1. Download COLx.tar.gz
2. sudo -s
3. gunzip COLx.tar.gz
4. tar -vxf COLx.tar
5. cd COLx
6. ./install.sh						    				-------  configures the file system and install the plugin


Usage
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1. Using terminal, move to the working directory containing your input files.
2. run COLx using command " gffex -pl colx -in [multi-columnfile] -cl [START column no.] [END column no.] -db [seqeuncefile]"		
												
											gffex -pl colx -in input -cl 9 10 -db seqeunce.fasta


Acknowledgements
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1. Dr. Dinesh Gupta
2. Mr. Abhinav Kaushik


DeveloperZ Information
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Achal Rastogi
(Dr. Dinesh Gutpa Group)
Research Fellow
Bioinformatics Facility
Structural & Computational Biology Laboratory
International Center for Genetic Engineering And Biotechnology
Aruna Asaf Ali Marg, New Delhi 110067 India
Email: achal@icgeb.res.in


NOTES
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1. Being in the directory containing input files is a prerequisite for running GFF-Ex, to avoid output errors.
2. Take care of the inputs, wrong inputs may fetch you with wrong results.
3. Remember to be super-user($ su) while installing. unistalling or executing GFF-Ex.