FETx ------------------------------------------------------------------------------ FETx is a plugin for GFF-Ex. FETx translates coordinates of user specified feature into sequences using GFF-Ex. System Requirements ------------------------------------------------------------------------------ 1. OS Platform:- Linux 2. Perl 4.0, or above 3. GFF-Ex_v2.1 Required Input Files ----------------------------------------------------------------------------- 1. Gff file (.gff) 2. Corresponding Genome Sequence file in fasta format. (.fa, .fasta, .fna, etc.) 3. feature of interest specified within gff file. Inputs Example Files ----------------------------------------------------------------------------- 1. Gff File: /FETx/examples/example.gff 2. Genome: /FETx/examples/sequence.fasta Output Files Preffix(plugin name)_Suffix(Input gfffile/sequencefile) ----------------------------------------------------------------------------- 1. Desired Sequences - OUTPUTFILE: fetx_(feature-name)_(input "genome" filename) Installation ----------------------------------------------------------------------------- 1. Download FETx.tar.gz 2. sudo -s 3. gunzip FETx.tar.gz 4. tar -vxf FETx.tar 5. cd FETx 6. ./install.sh ------- configures the file system and install the plugin Usage ----------------------------------------------------------------------------- 1. Using terminal, move to the working directory containing your input files. 2. run FETx using command " gffex -pl [fetx] -in [gff-file] -db [seqeuncefile] -ft featurename" gffex -pl fetx -in input -db seqeunce.fasta -ft gene Acknowledgements ------------------------------------------------------------------------------ 1. Dr. Dinesh Gupta 2. Mr. Abhinav Kaushik DeveloperZ Information ------------------------------------------------------------------------------ Achal Rastogi (Dr. Dinesh Gutpa Group) Research Fellow Bioinformatics Facility Structural & Computational Biology Laboratory International Center for Genetic Engineering And Biotechnology Aruna Asaf Ali Marg, New Delhi 110067 India Email: achal@icgeb.res.in NOTES ------------------------------------------------------------------------------ 1. Being in the directory containing input files is a prerequisite for running GFF-Ex, to avoid output errors. 2. Take care of the inputs, wrong inputs may fetch you with wrong results. 3. Remember to be super-user($ su) while installing. unistalling or executing GFF-Ex. 4. Scripts mounted and tested on Red-Hat EL 5.1.