System Requirements ------------------------------------------------------------------------------ 1. OS Platform:- Linux 2. Perl 4.0, or above Required Inputs ----------------------------------------------------------------------------- 1. Gff file (.gff) 2. Corresponding Genome file in fasta format. Inputs Example Files ----------------------------------------------------------------------------- 1. Gff File: /GFF/examples/example.gff 2. Genome: /GFF/examples/sequence.fasta Output Preffix(Feature Name)_Suffix(Input GFF/Genome) ----------------------------------------------------------------------------- 1. gff file wth unique ID i.e their Sr. Nos. - OUTPUT: ID_(input "gff" filename) 1. Gene Sequences - OUTPUT: gene_(input "genome" filename) 2. Exon Sequences - OUTPUT: exon_(input "genome" filename) 3. CDS/cDNA Sequences - OUTPUT: CDS_(input "genome" filename) 4. mRNA Sequences - OUTPUT: mRNA_(input "genome" filename) 5. Intron Sequences - OUTPUT: intron_(input "genome" filename) 6. Intergenic Sequences - OUTPUT: intergenic_(input "genome" filename) 7. Promoter / Gene Upstream Sequences - OUTPUT: upstream_(input "genome" filename) Note: The output sequences will be in fasta format and the header of each seqeunce will state the Serial Numbers or Unique IDs preffixed with the plugin name (eg. gene_20). Unique IDs/Serial Numbers will be in accordance to the IDs/Serial Numbers (first column) in the Unique-ID file [OUTPUTFILE: ID_(input "gff" filename]. Installation ----------------------------------------------------------------------------- 1. Download GFF_v2.0.tar.gz 2. sudo -s 3. gunzip GFF_v2.0.tar.gz 4. tar -vxf GFF_2.0.tar 5. cd GFF_v2.0 6. ./install.sh ------- configures the file system and install the program Unstallation ----------------------------------------------------------------------------- 1. cd $PWD/GFF 2. ./unistall Usage ----------------------------------------------------------------------------- 1. Using terminal, move to the working directory containing your input files. 2. run Gff-Ex. using command " gff " and follow the instructions. gff Acknowledgements ------------------------------------------------------------------------------ 1. Dr. Dinesh Gupta 2. Mr. Abhinav Kaushik DeveloperZ Information ------------------------------------------------------------------------------ Achal Rastogi (Dr. Dinesh Gutpa Group) Junior Research Fellow Bioinformatics Facility Structural & Computational Biology Laboratory International Center for Genetic Engineering And Biotechnology Aruna Asaf Ali Marg, New Delhi 110067 India Email: achal@icgeb.res.in NOTES ------------------------------------------------------------------------------ 1. Works best with gff files in GFF-3 format. 2. Being in the directory containing input files is a prerequisite for running GFF-Ex, to avoid output errors. 3. Remember to be super-user($ su) while installing. unistalling or executing GFF-Ex.