Malaria is one of the major global health concerns still prevailing in this 21st century. Even the effect of artemisinin combination therapies (ACT) have declined and causing more mortality across the globe. Therefore, it is important to understand the basic biology of malaria parasite in order to find novel drug targets. Helicases play important role in\u00a0nucleic acid metabolism<\/a>\u00a0and are components of cellular machinery in various organisms. In this manuscript we have performed the biochemical characterization of homologue of\u00a0DDX17<\/a>\u00a0from\u00a0Plasmodium falciparum<\/a><\/em>\u00a0(PfDDX17). Our results show that PfDDX17 is an active RNA helicase and uses mostly ATP for its function. The qRT-PCR experiment results suggest that PfDDX17 is highly expressed in the trophozoite stage and it is localised mainly in the cytoplasm and in infected RBC (iRBC) membrane mostly in the trophozoite stage. The dsRNA knockdown study suggests that PfDDX17 is important for\u00a0cell cycle progression<\/a>. These studies report the biochemical functions of PfDDX17 helicase and further augment the fundamental knowledge about helicase families of\u00a0P. falciparum<\/em>.<\/p>\n